Tag Archives: Royal Society

500th post

Map of all readership distributionThis is the five hundredth post on this blog.  The first 21 posts were published randomly between July 11th, 2012, and January 4th, 2013; and the weekly posts only started on January 7th, 2013, so I have another 48 posts to publish before I can claim a decade of weekly posts.  Nevertheless, I feel it is worth shouting about 500 posts.

I am a little surprised to realise that I have written five hundred posts and it has made me pause to think about why I write them.  A number of answers came to mind, including because I enjoy writing – it empties my mind and allows me to move on to new thoughts or, on other occasions, it allows me to arrange my thoughts into some sort of order.  I also write posts to communicate ideas, to disseminate research, to entertain and to fulfill a commitment, initially to funding bodies (I started the blog as part of commitment to Royal Society Wolfson Research Merit Award) but increasingly to readers of the blog.  I am amazed that for the last five years the blog has been read in more 140 countries.  While I have a handful of statistics about the readership, beyond the small handful of readers who correspond with me or who I meet in person, I have no idea who reads the blog.  Most of time I do not give much thought to who is reading my posts and my intended reader is a rather vague fuzzy figure who barely exists in my mind.

The map shows the distribution of all readers over the 500 posts with the darker colour indicating more readers per country.

Nano biomechanical engineering of agent delivery to cells

figure 1 from [1] with text explanationWhile many of us are being jabbed in the arm to deliver an agent that stimulates our immune system to recognize the coronavirus SARS-CoV-2 as a threat and destroy it, my research group has been working, in collaboration with colleagues at the European Commission Joint Research Centre, on the dynamics of nanoparticles [1] [see ‘Size matters‘ on October 23rd, 2019] which could be used as carriers for the targeted delivery of therapeutic, diagnostic and imaging agents in the human body [2].  The use of nanoparticles to mechanically stimulate stem cells to activate signalling pathways and modulate their differentiation also has some potential [3]. In studies of the efficacy of nanoparticles in these biomedical applications, the concentration of nanoparticles interacting with the cell is a primary factor influencing both the positive and negative effects.  Such studies often involve exposing a monolayer of cultured cells adhered to the bottom of container to a dose of nanoparticles and monitoring the response over a period of time.  Often, the nominal concentration of the nanoparticles in biological medium supporting the cells is reported and used as the basis for determining the dose-response relationships.  However, we have shown that this approach is inaccurate and leads to misleading results because the nanoparticles in solution are subject to sedimentation due to gravity, Brownian motion [see ‘Slow moving nanoparticles‘ on December 13th, 2017] and inter-particle forces [see ‘ Going against the flow‘ on February 3rd, 2021] which affect their transport within the medium [see graphic] and the resultant concentration adjacent to the monolayer of cells.  Our experimental results using the optical method of caustics [see ‘Holes in fluids‘ on October 22nd, 2014] have shown that nanoparticle size, colloidal stability and solution temperature influence the distribution of nanoparticles in solution.  For particles larger than 60 nm in diameter (about one thousandth of the diameter of a human hair) the nominal dose differs significantly from the dose experienced by the cells.  We have developed and tested a theoretical model that accurately describes the settling dynamics and concentration profile of nanoparticles in solution which can be used to design in vitro experiments and compute dose-response relationships.

References

[1] Giorgi F, Macko P, Curran JM, Whelan M, Worth A & Patterson EA. 2021 Settling dynamics of nanoparticles in simple and biological media. Royal Society Open Science, 8:210068.

[2] Daraee H, Eatemadi A, Abbasi E, Aval SF, Kouhi M, & Akbarzadeh A. 2016 Application of gold nanoparticles in biomedical and drug delivery. Artif. Cells Nanomed. Biotechnol. 44, 410–422. (doi:10.3109/21691401.2014.955107)

[3] Wei M, Li S, & Le W. 2017 Nanomaterials modulate stem cell differentiation: biological
interaction and underlying mechanisms. J. Nanobiotechnol. 15, 75. (doi:10.1186/s12951-
017-0310-5)

From strain measurements to assessing El Niño events

Figure 11 from RSOS 201086One of the exciting aspects of leading a university research group is that you can never be quite sure where the research is going next.  We published a nice example of this unpredictability last week in Royal Society Open Science in a paper called ‘Transformation of measurement uncertainties into low-dimensional feature space‘ [1].  While the title is an accurate description of the contents, it does not give much away and certainly does not reveal that we proposed a new method for assessing the occurrence of El Niño events.  For some time we have been working with massive datasets of measurements from arrays of sensors and representing them by fitting polynomials in a process known as image decomposition [see ‘Recognising strain‘ on October 28th, 2015]. The relatively small number of coefficients from these polynomials can be collated into a feature vector which facilitates comparison with other datasets [see for example, ‘Out of the valley of death into a hype cycle‘ on February 24th, 2021].  Our recent paper provides a solution to the issue of representing the measurement uncertainty in the same space as the feature vector which is roughly what we set out to do.  We demonstrated our new method for representing the measurement uncertainty by calibrating and validating a computational model of a simple beam in bending using data from an earlier study in a EU-funded project called VANESSA [2] — so no surprises there.  However, then my co-author and PhD student, Antonis Alexiadis went looking for other interesting datasets with which to demonstrate the new method.  He found a set of spatially-varying uncertainties associated with a metamodel of soil moisture in a river basin in China [3] and global oceanographic temperature fields collected monthly over 11 years from 2002 to 2012 [4].  We used the latter set of data to develop a new technique for assessing the occurrence of El-Niño events in the Pacific Ocean.  Our technique is based on global ocean dynamics rather than on the small region in the Pacific Ocean which is usually used and has the added advantages of providing a confidence level on the assessment as well as enabling straightforward comparisons of predictions and measurements.  The comparison of predictions and measurements is a recurring theme in our current research but I did not expect it to lead into ocean dynamics.

Image is Figure 11 from [1] showing convex hulls fitted to the cloud of points representing the uncertainty intervals for the ocean temperature measurements for each month in 2002 using only the three most significant principal components . The lack of overlap between hulls can be interpreted as implying a significant difference in the temperature between months.

References:

[1] Alexiadis, A. and Ferson, S. and  Patterson, E.A., , 2021. Transformation of measurement uncertainties into low-dimensional feature vector space. Royal Society Open Science, 8(3): 201086.

[2] Lampeas G, Pasialis V, Lin X, Patterson EA. 2015.  On the validation of solid mechanics models using optical measurements and data decomposition. Simulation Modelling Practice and Theory 52, 92-107.

[3] Kang J, Jin R, Li X, Zhang Y. 2017, Block Kriging with measurement errors: a case study of the spatial prediction of soil moisture in the middle reaches of Heihe River Basin. IEEE Geoscience and Remote Sensing Letters, 14, 87-91.

[4] Gaillard F, Reynaud T, Thierry V, Kolodziejczyk N, von Schuckmann K. 2016. In situ-based reanalysis of the global ocean temperature and salinity with ISAS: variability of the heat content and steric height. J. Climate. 29, 1305-1323.

Out of the valley of death into a hype cycle?

Fig 5 from Middleton et al with full captionThe capability to identify damage and track its propagation in structures is important in ensuring the safe operation of a wide variety of engineering infrastructure, including aircraft structures. A few years ago, I wrote about research my group was performing, in the INSTRUCTIVE project [see ‘INSTRUCTIVE final reckoning‘ on January 9th, 2019] with Airbus and Strain Solutions Limited, to deliver a new tool for monitoring the development of damage using thermoelastic stress analysis (TSA) [see ‘Counting photons to measure stress‘ on November 18th, 2015].  We collected images using a TSA system while a structural component was subject to cycles of load that caused damage to initiate and propagate during a fatigue test. The series of images were analysed using a technique based on optical flow to identify apparent movement between the images which was taken as indication of the development of damage [1]. We demonstrated that our technique could indicate the presence of a crack less than a millimetre in length and even identify cracks initiating under the heads of bolts using experiments performed in our laboratory [see ‘INSTRUCTIVE update‘ on October 4th, 2017].  However, this technique was susceptible to errors in the images when we tried to use low-cost sensors and to changes in the images caused by flight cycle loading with varying amplitude and frequency of loads.  Essentially, the optical flow approach could be fooled into identifying damage propagation when a sensor delivered a noisy image or the shape of the load cycle was changed.  We have now overcome this short-coming by replacing the optical flow approach with the orthogonal decomposition technique [see ‘Recognising strain‘ on October 28th, 2015] that we developed for comparing data fields from measurements and predictions in validation processes [see ‘Million to one‘ on November 21st, 2018] .  Each image is decomposed to a feature vector and differences between the feature vectors are indicative of damage development (see schematic in thumbnail from [2]).  The new technique, which we have named the differential feature vector method, is sufficiently robust that we have been able to use a sensor costing 1% of the price of a typical TSA system to identify and track cracks during cyclic loading.  The underpinning research was published in December 2020 by the Royal Society [2] and the technique is being implemented in full-scale ground-tests on aircraft structures as part of the DIMES project.  Once again, a piece of technology is emerging from the valley of death [see ‘Slowly crossing the valley of death‘ on January 27th, 2021] and, without wishing to initiate the hype cycle [see ‘Hype cycle‘ on September 23rd, 2015], I hope it will transform the use of thermal imaging for condition monitoring.

Logos of Clean Sky 2 and EUThe INSTRUCTIVE and DIMES projects have received funding from the Clean Sky 2 Joint Undertaking under the European Union’s Horizon 2020 research and innovation programme under grant agreements No. 685777 and No. 820951 respectively.

The opinions expressed in this blog post reflect only the author’s view and the Clean Sky 2 Joint Undertaking is not responsible for any use that may be made of the information it contains.

References

[1] Middleton CA, Gaio A, Greene RJ & Patterson EA, Towards automated tracking of initiation and propagation of cracks in Aluminium alloy coupons using thermoelastic stress analysis, J. Non-destructive Testing, 38:18, 2019.

[2] Middleton CA, Weihrauch M, Christian WJR, Greene RJ & Patterson EA, Detection and tracking of cracks based on thermoelastic stress analysis, R. Soc. Open Sci. 7:200823, 2020.