Tag Archives: bacteria

Label-free real-time tracking of individual bacterium

Images from the optical microscope showing the tracks of bacteria interacting with a surfaceAntimicrobial resistant (AMR) infections are already the third leading cause of death in the USA and are predicted to kill 50 million people per year by 2050.  It is the next pandemic starting already.  We have been using our capability to track nanoparticles in an optical microscope [see ‘Slow moving nanoparticles‘ on December 13th, 2017 and ‘Nano biomechanical engineering of agent delivery to cells‘ on December 15th, 2021] to track individual bacterium as they interact with surfaces to form biofilms.  Bacterial biofilms are complex colonies of bacteria that are highly resistant to antimicrobial agents and can cause life-threatening infections.  We have used our label-free, real-time tracking capabilities to explore the dynamics and adhesion of bacteria to surfaces and found that viable bacteria adhered to the surface but continue to move with rotary or sliding motions depending on the mechanics of their attachment to the surface.  Bacteria that were killed by contact with the surface did not move once they were attached to the surface.  The image shows examples of these motions from our paper published last month.  Our ability to detect these differences in the dynamics of bacteria will allow us to detect the onset of the formation of biofilms and to quantify the efficacy of antimicrobial surfaces and coatings.

Image: Figure 4 – Tracks (yellow lines) of the sections (purple circles) of four E. coli bacteria experiencing: (a) random diffusion above the surface; (b) rotary attachment; (c) lateral attachment; (d) static attachment. The dynamics of the four bacteria was monitored for approximately 20 s. The length of the scale bars is 5 μm. From Scientific Reports, 12:18146, 2022.

Source:

Giorgi F, Curran JM & Patterson EA, Real-time monitoring of the dynamics and interactions of bacteria and the early-stage formation of biofilms, Scientific Reports, 12:18146, 2022.

Fancy a pint of science?

In September I am planning to initiate a new research project on the interaction of bacteria with cellular and hard surfaces.  It is in collaboration with Jude Curran and is co-funded by Unilever and the Biotechnology and Biological Sciences Research Council.  We have already used the optical method of caustics in a microscope to track and characterise the movement of synthetic nanoparticles as small as 3 nm in an array of biologically-relevant solutions [see ‘Nano biomechanical engineering of agent delivery to cells’ on December 15th, 2021].  We have also used the same technique to characterise and quantify the motion and growth of bacteria in solutions.  Now, we plan to use caustic signatures as a label-free tracking technology for pre-clinical testing of antimicrobial solutions and coatings.  We plan to start by considering binding and removal of viral particles and bacterial spores from hard and soft laundry surfaces using various bacterial species, including Staph aureus which is responsible for laundry malodour; before progressing to the interaction of bacteria with human oral and skin cell cultures.  We are in the process of recruiting a suitable PhD student so if you are interested or know someone who might be suitable then get in touch.  If you want to learn more about our tracking technology and fancy a pint of science, then join us in Liverpool in May for part of the world’s largest festival of public science.  I will be talking about ‘Revealing the invisible: real-time motion of virus particles’  on May 10th at 7.30pm on Leaf of Bold Street.

Liverpool Pint of Science programme

UK Pint of Science programme

 

Follow your gut

Decorative image of a fruit fly nervous system Albert Cardona HHMI Janelia Research Campus Welcome Image Awards 2015Data centres worldwide consume about 1% of global electricity generation, that’s 200-250 TWh (Masenet et al, 2020), and if you add in mining of cryptocurrencies then consumption jumps by about 50% (Gallersdörfer et al, 2020). Data transmission consumes about 260-340 TWh or at least another 1% of global energy consumption (IEA, 2020).  The energy efficiency of modern computers has been improving; however, their consumption is still many millions times greater than the theoretical limit defined by Landauer’s principle which was verified in 2012 by Bérut et al.  According to Landauer’s principle, a computer operating at room temperature would only need 3 zJ (300 billion billionths of a Joule) to erase a bit of information.  The quantity of energy used by modern computers is many millions times the Landauer limit.  Of course, progress is being made almost continuously, for example a team at EPFL in Lausanne and ETH Zurich recently described a new technology that uses only a tenth of the energy of current transistors (Oliva et al 2020).  Perhaps we need turn to biomimetics because Escherichia Coli, which are bacteria that live in our gut and have to process information to reproduce, have been found to use ten thousand times less energy to process a bit of information than the average human-built device for processing information (Zhirnov & Cavin, 2013).  So, E.coli are still some way from the Landauer limit but demonstrate that there is considerable potential for improvement in engineered devices.

References

Bérut A, Arakelyan A, Petrosyan A, Ciliberto S, Dillenschneider R & Lutz E. Experimental verification of Landauer’s principle linking information and thermodynamics. Nature, 483: 187–189, 2012.

IEA (2021), Data Centres and Data Transmission Networks, IEA, Paris https://www.iea.org/reports/data-centres-and-data-transmission-networks

Gallersdörfer U, Klaaßen L, Stoll C. Energy consumption of cryptocurrencies beyond bitcoin. Joule. 4(9):1843-6, 2020.

Masanet E, Shehabi A, Lei N, Smith S, Koomey J. Recalibrating global data center energy-use estimates. Science. 367(6481):984-6, 2020.

Oliva N, Backman J, Capua L, Cavalieri M, Luisier M, Ionescu AM. WSe 2/SnSe 2 vdW heterojunction Tunnel FET with subthermionic characteristic and MOSFET co-integrated on same WSe 2 flake. npj 2D Materials and Applications. 4(1):1-8, 2020.

Zhirnov VV, Cavin RK. Future microsystems for information processing: limits and lessons from the living systems. IEEE Journal of the Electron Devices Society. 1(2):29-47, 2013.

My Engineering Day

Photograph of roof tops and chimneys in Liverpool.Today is ‘This is Engineering’ day organised by the Royal Academy of Engineering to showcase what engineers and engineering really look like, celebrate our impact on the world and shift public perception of engineering towards an appreciation that engineers are a varied and diverse group of people who are critical to solving societal challenges.  You can find out more at https://www.raeng.org.uk/events/online-events/this-is-engineering-day-2020.  I have decided to contribute to ‘This is Engineering’ day by describing what I do on a typical working day as an engineer. 

Last Wednesday was like many other working days during the pandemic.  I got up about 7am went downstairs for breakfast in our kitchen and then climbed back upstairs to my home-office in the attic of our house in Liverpool [see ‘Virtual ascent of Moel Famau’ on April 8th, 2020].  I am lucky in that my home-office is quite separate from the living space in our house and it has a great view over the rooftops.  I arrived there at about 7.45am, opened my laptop, deleted the junk email, and dealt with the emails that were urgent, interesting or could be replied to quickly.  At around 8am, I closed my email and settled down to write the first draft of a proposal for funding to support our research on digital twins [see ‘Tacit hurdle to digital twins’ on August 26th, 2020].  I had organised a meeting earlier in the week with a group of collaborators and now I had the task of converting the ideas from our discussion into a coherent programme of research.  Ninety caffeine-fuelled minutes later, I had to stop for a Google Meet call with a collaborator at Airbus in Toulouse during which we agreed the wording on a statement about the impact our recent research efforts.  At 10am I joined a Skype call for a progress review with a PhD student on our dual PhD programme with National Tsing Hua University in Taiwan, so we were joined by his supervisor in Taiwan where it was 6pm [see ‘Citizens of the World’ on November 27th, 2019].  The PhD student presented some very interesting results on evaluating the waviness of fibres in carbon-fibre composite materials using ultrasound measurements which he had performed in our laboratory in Liverpool.  Despite the local lockdown in Liverpool due to the pandemic, research laboratories on our campus are open and operating at reduced occupancy to allow social distancing.

After the PhD progress meeting, I had a catch-up session with my personal assistant to discuss my schedule for the next couple of weeks before joining a MS-Teams meeting with a couple of colleagues to discuss the implications of our current work on computational modelling and possible future directions.  The remaining hour up to my lunch break was occupied by a conference call with a university in India with whom we are exploring a potential partnership.  I participated in my capacity as Dean of the School of Engineering and joined about twenty colleagues from both institutions discussing possible areas of collaboration in both research and teaching.  Then it was back downstairs for a half-hour lunch break in the kitchen. 

Following lunch, I continued in my role as Dean with a half-hour meeting with Early Career Academics in the School of Engineering followed by internal interviews for the directorship of one of our postgraduate research programmes.  At 3.30pm, I was able to switch back to being a researcher and meet with a collaborator to discuss the prospects for extending our work on tracking synthetic nanoparticles into monitoring the motion of biological entities such as viruses and bacteria [see ‘Modelling from the cell through the individual to the host population’ on May 5th 2020].  Finally, as usual, I spent the last two to three hours of my working day replying to emails, following up on the day’s meetings and preparing for the following day.  One email was a request for help from one of my PhD students working in the laboratory who needed a piece of equipment that had been stored in my office for safekeeping.  So, I made the ten-minute walk to campus to get it for her which gave me the opportunity to talk face-to-face with one of the post-doctoral researchers in my group who is working on the DIMES project [see ‘Condition-monitoring using infra imaging‘ on June 17th, 2020].  After dinner, my wife and I walked down to the Albert Dock and along the river front to Princes Dock and back up to our house.

So that was my Engineering Day last Wednesday!

 

Logos of Clean Sky 2 and EUThe DIMES project has received funding from the Clean Sky 2 Joint Undertaking under the European Union’s Horizon 2020 research and innovation programme under grant agreement No. 820951.  The opinions expressed in this blog post reflect only the author’s view and the Clean Sky 2 Joint Undertaking is not responsible for any use that may be made of the information it contains.